sapsan
Joined 11 years ago
Karma
77
Submissions
35
Member since
Jan 31
Recent submissions
Comment · 24 days ago
W&B -> CoreWeave
Comment · 2 years ago
I recently saw this about AlphaFold: https://elanapearl.github.io/blog/2024/the-illustrated-alpha....
I don't think it's going to answer all your question but it might still help!
Comment · 3 years ago
Comment · 3 years ago
I have been enjoying https://oku.club/ recently in case anyone is searching for a nicely functioning alternative.
Comment · 4 years ago
I believe the parent refers to this [1,2,3] study. Indeed, this was about targeting many (11,923) genes with Perturb-seq (CRISPR screen with single-cell RNA-sequencing readout). There are two human cell lines used in the study (K562 and RPE1). For functional annotation, authors focused on 1,973 targeted genes that had strong transcriptional phenotype after the perturbation. As there's some correlation structure, that's what they studied, annotating clusters of individual perturbations using public databases (like STRING [4]) and literature. Seems like a lot of great work has been done here though stating that we now know all the functions of all the genes might be a bit of a stretch indeed.
[1]: https://news.mit.edu/2022/crispr-based-map-ties-every-human-... [2]: https://www.cell.com/cell/fulltext/S0092-8674(22)00597-9 [3]: https://gwps.wi.mit.edu/ [4]: https://string-db.org/
Comment · 4 years ago
Seems to be an immortalized (telomerase*-transformed) cell line from a female fetus with near-complete homozygosity (https://sites.google.com/ucsc.edu/t2tworkinggroup/chm13-cell...).
* Telomerase is a reverse transcriptase that allows to achieve replicative immortality (https://academic.oup.com/hmg/article/9/3/403/715108).
Comment · 5 years ago
Sounds similar to the consequences of reading Don Norman's «The Design of Everyday Things»...