rafaelferreira

> To produce the protein needed for insulin, we need to grow microorganisms with a bioreactor and purify the protein from the culture with a protein purification system (FPLC). Proprietary examples of this equipment come at a very high initial cost and with high ongoing costs of support from the manufacturers. Our goal is to develop easy to manage, easy to repair, and affordable equipment to sustain local and community-built insulin production. > Our FPLC design is in the early stages, and we are steadily developing mechanical and electrical designs to detect UV-C absorbance and manage the concentration of two buffers via peristaltic pumps and a mixing chamber. We plan to use Arduino microcontrollers as well as the Raspberry Pi, and the finished device will make use of the open-source Ender 3 3D Printer to facilitate automated fraction collection with G-CODE input. The bioreactor design makes use of quite a few commercial off-the-shelf parts and is ready for prototyping.

https://www.youtube.com/watch?v=MnrRUdbOW1M