Ah, brings me back to the countless nights I spent counting plate after plate of HEK293 cells using a Haemocytometer [0], a light microscope, and a mechanical counter [1].
At least with HEK293 cells you could mostly tell if they were dead through the microscope (dead cells are darker).
At least with HEK293 cells you could mostly tell if they were dead through the microscope (dead cells are darker).
[0] https://en.wikipedia.org/wiki/Hemocytometer
[1] https://en.wikipedia.org/wiki/Tally_counter