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When I did the same, also 15 years ago, the lab on campus would only Sanger sequence my insert - and for targets larger than ~600bp I also had to include internal sequencing primers (that had to be ordered separately, shipped, etc). Plasmidsaurus will return the entire plasmid sequence so you can verify it is intact, you don't have to provide any internal sequencing primers, simple repeats will be more accurately sequenced, and you can just pop some tubes in a padded mailer in the post at room temperature.

I'm no longer doing wet bench work but Plasmidsaurus seems like a service I'd happily use.

I didn't read the entire document in depth, but perhaps it has been shown elsewhere that first year GPA is a good quality predictor of GPA in subsequent years.

Were your heater and cooler equally efficient?

The article is less about that but more about the need for clear policies and consistent adherence to said policies regarding plus sized passengers.

Thank you for sharing. This was one of the first funny videos I downloaded from the internet.

Yes and no. I enjoy getting personal tasks done in the morning before work, especially those that require a sharp mind. Like you mentioned, I feel worn out mentally and the end of the day, though I find I'm happier and more productive overall if I save a few sharp-minded hours in the early morning for myself.

People choose to be tested for Huntington disease, including many young adults who have recently turned 18 (in the USA). While, like yourself, I don't know what I would personally chose to do if faced with this situation, the availability of a specific test with a high NPV and low FP rate would be beneficial to have.

The only solution I can think of is for myself to buy a lifted truck or other similar unnecessarily large vehicle. I haven't done this, of course, but if I did I'd then be part of the problem but at least my eye line would be above that of the majority of other drivers. We have a sedan and a small SUV - I don't drive the sedan at night ever because of the headlight problem. My eyes hurt just thinking about this.

Mammalian cells in research labs are grown in media that is composed of 1) multiple powdered components that are readily obtainable through isolation from bacteria/yeast or synthesis and, 2) 10% FBS (fetal bovine serum). Until these cultured meat companies can come up with a suitable replacement for FBS, due to environmental, moral, cost, etc concerns, none of these solutions are terribly viable. I personally wouldn't want to eat (or pay for) a tiger streak that required liters of FBS to grow.

I'm wondering how they plan to get decent content. I just searched two of my interests, climbing and canoeing, and only a handful of uninteresting videos popped up. Are there specific topics that have engaging communities on the site?

The media is primarily reporting good news instead of actual news. Twitter has been the best source of images, videos, and descriptions of what is happening on the ground. Search #deprem (earthquake in Turkish) to see for yourself.

This type of data is being collected at large academic institutions who are doing longitudinal analyses of their patient population.

Exacrly, they would say the test detected a pathogenic variant in the SHH gene.

I would prefer unprocessed food as well but it sounds like cost is a major driver in this case.