The high-percentage cause is that the transfer was actually sent and it is stuck in processing at your bank. This actually happens to a non-zero percentage of transactions, and this is OK, because they get investigated and recovered by CS teams. (Banks, surprisingly, aren't
actually ACID even though everyone uses them as the canonical example of an ACID application.)
Escalate with your bank. You do not want to speak to Tier 1 telephone CS on this. You want to speak to someone in their wires investigation department, or to someone who can escalate a call to them. A very quick way to achieve this is to go to the local branch, ask to see one of their business representatives, and explain the situation then sit while they resolve things. If they get the runaround, you ask for the branch manager, mention $10k+ is on the line, and he escalates for you.
Another example is calling directly into the wire investigation group. Now that you know it exists, you can probably figure out how to find it on Google. (If you can't find their group call the Wealth Management group and say "Hiya, someone told me to call you guys. $BIGCO made an incoming wire to my $MYBANK account and it has gone missing. Can you trace it for me?" then wait while they either do so or transfer you.)
Beyond the instant problem with the wire transfer, which is unfortunate, please allow me the liberty of giving unsolicited business advice. It appears that this situation rates "emergency" for your business. That should never happen with regards to one single payment, because routine business mishaps (like, e.g., a bank delaying a payment) will routinely cause events like this to happen.
There are a variety of ways to control for your risk for this. Two which you should implement, as soon as reasonably practical, are a) building up a savings buffer such that you're not dependent on your cashflow to eat and b) charging more, which is partially justified by risks you absorb from having a freelancer/consultant/etc charging model and not being a W-2 employee who could walk to Payroll and say "I don't care what the problem is, just fix it" and having the expectation that Payroll would leap to fix that.
Escalate with your bank. You do not want to speak to Tier 1 telephone CS on this. You want to speak to someone in their wires investigation department, or to someone who can escalate a call to them. A very quick way to achieve this is to go to the local branch, ask to see one of their business representatives, and explain the situation then sit while they resolve things. If they get the runaround, you ask for the branch manager, mention $10k+ is on the line, and he escalates for you.
Another example is calling directly into the wire investigation group. Now that you know it exists, you can probably figure out how to find it on Google. (If you can't find their group call the Wealth Management group and say "Hiya, someone told me to call you guys. $BIGCO made an incoming wire to my $MYBANK account and it has gone missing. Can you trace it for me?" then wait while they either do so or transfer you.)
Beyond the instant problem with the wire transfer, which is unfortunate, please allow me the liberty of giving unsolicited business advice. It appears that this situation rates "emergency" for your business. That should never happen with regards to one single payment, because routine business mishaps (like, e.g., a bank delaying a payment) will routinely cause events like this to happen.
There are a variety of ways to control for your risk for this. Two which you should implement, as soon as reasonably practical, are a) building up a savings buffer such that you're not dependent on your cashflow to eat and b) charging more, which is partially justified by risks you absorb from having a freelancer/consultant/etc charging model and not being a W-2 employee who could walk to Payroll and say "I don't care what the problem is, just fix it" and having the expectation that Payroll would leap to fix that.