Comment by BSPtree

I would not think so, at least not in a meaningful way. That would require to edit the cartridge data thus the ROM, as all sprite data was contained within the cartridge. Sample based sound is impossible natively, as NES and older game consoles don't work with digital data but synthesizers. You could perhaps synthesize the music to WAV files and then enchance it. But then again, NES and thus its emulators have no way of playing digitized audio!