I really need to do a write-up. I kinda just whip up the easiest path and do it.
For example for the grape, I needed to knock out some tryptophan synthesis genes so I could redirect the bioflux. Problem is that in bakers yeast they have a whole buncha copies of their chromosomes, so I had to knock out one of the genes and replace it with a different gene from grapes. Did that with a quick lil CRISPR switch.
Had to electroporate tho because the transformation rates on wild/bakers/non-lab yeast are so garbage
For example for the grape, I needed to knock out some tryptophan synthesis genes so I could redirect the bioflux. Problem is that in bakers yeast they have a whole buncha copies of their chromosomes, so I had to knock out one of the genes and replace it with a different gene from grapes. Did that with a quick lil CRISPR switch.
Had to electroporate tho because the transformation rates on wild/bakers/non-lab yeast are so garbage