I’m referring to the experiment done in the OP - the most I’ve read about from an minION flow cell is 8 Gb (and this is from cell line preps with tons of DNA, so the coverage isn’t great).
You need multiple flow cells or a higher capacity flow cell to get anything close to 1X on an unselected genome prep.
Shotgun sequencing isn’t probably what you meant to say - this is all enzymatic or, if it’s sonicated, gets size selected.
You need multiple flow cells or a higher capacity flow cell to get anything close to 1X on an unselected genome prep.
Shotgun sequencing isn’t probably what you meant to say - this is all enzymatic or, if it’s sonicated, gets size selected.