I've been learning a lot about molecular biology recently, and am about to run some procedures related to plasmid isolation, gene cloning, and expression. Of note, the resources I've read, from AddGene, and The Bumbling Biochemist (excellent Youtube channel) emphasize sending your plasmids in for sequencing once complete, due to all the errors that can occur.
I am wondering if the plasmids the researchers here tested simply weren't sequenced. This wouldn't explain all classes of errors (like toxic protein production), but it may explain some. When you order from AddGene, they provide both the depositor's sequence results, if available, and their own results. The paper doesn't mention AddGene specifically; I'm curious how their plasmids compare to the ones tested.
Regarding toxic proteins: It seems like that would be a straightforward software addition to pass sequencing results through.
I am wondering if the plasmids the researchers here tested simply weren't sequenced. This wouldn't explain all classes of errors (like toxic protein production), but it may explain some. When you order from AddGene, they provide both the depositor's sequence results, if available, and their own results. The paper doesn't mention AddGene specifically; I'm curious how their plasmids compare to the ones tested.
Regarding toxic proteins: It seems like that would be a straightforward software addition to pass sequencing results through.