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Comment by sierra1011

I used to work in a genetics lab, and had to prepare trays of gel to visualise DNA tests (PCR testing to identify HLA alleles: effectively like finding someone's blood type, but for their immune system).

The compound in the gel that attaches itself to the DNA particles and makes it so we can visualise the results by UV fluorescence is Ethidium Bromide [1], one of the lower state Bromine salts the article mentions.

The way the electrophoresis gels are (were, by me and the other workers) prepared is by... microwaving a flask with it in. Repeatedly taking it out as it gets hotter to mix it. Usually involving some release of steam. Which includes a small amount of the bromide. Fine in small doses, but when you do it multiple times a day, 5 days a week, 45 weeks of the year...

To this day I wonder how much of an effect it's going to have on me and my colleagues at the time.

[1] https://en.m.wikipedia.org/wiki/Ethidium_bromide

parent sierra1011 2 years ago

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