Comment by abhisuri97

Hey, lowly Bio undergrad here, but how are you able to skip the RNA extraction step? I read the paper and you use the viral transport medium, but wouldn't you have to also purify RNA from that (or is it just much easier to extract RNA from that medium)? I also dived into the paper behind the "skip the RNA extraction step" methodology and it basically seems to swap out one RNA extraction kit for another (Qiagen RNeasy Mini kit and the Qiagen RNeasy Micro kit). Couldn't shifting kits from one provider to another introduce supply chain strain? (or am I just oversimplifying it?)